Karla Apgar
Frederick
Summary
Skilled and dedicated Cytogenetics Technologist with 3 years of experience in performing cytogenetic analyses such as Fluorescence in situ hybridization (FISH) for diagnostic and research purposes. Proficient in processing and analyzing patient samples, maintaining stringent quality control standards, and ensuring compliance with regulatory requirements. Experienced in utilizing advanced laboratory technologies and methodologies to support genetic diagnostics and contribute to accurate, life-changing results for patients. Eager to expand my expertise into broader genetic research roles, contributing to innovative advances in genomics, precision medicine, and personalized healthcare.
Overview
7
7
years of professional experience
Work History
Cytogenetics Technologist II
GeneDx
03.2023 - Current
- Process CVs, Products of conceptions (POC) samples, blood harvests, and perform FISH hybridizations for accurate genetic analysis and diagnostic purposes.
- Develop new training materials and assist in the revision of Standard Operating Procedures (SOPs) to improve operational efficiency and ensure compliance with industry standards.
- Prepare and optimize culture media for various cell lines such as amniocytes, fibroblasts, villi, while ensuring proper nutrition and growth conditions.
- Prepare and maintain laboratory equipment, ensuring all instruments are calibrated and operational.
- Label, and organize samples while following established protocols.
- Ensure proper disposal of hazardous materials following OSHA guidelines.
- Train and mentor new lab assistants on lab safety protocols and standard laboratory procedures to ensure compliance with industry standards and maintain a safe working environment.
- Coordinate inventory management for supplies and equipment, ensuring optimal stock levels to maximize team efficiency and support seamless laboratory operations.
- Conduct comprehensive system analysis to identify areas for improvement, leading to more streamlined operations and reduced costs.
- Manage and optimize the use of the LIMS to track sample lifecycle, including intake, testing, and disposal, ensuring that all data is accurately logged.
Cytogenetics Technician I & II
GeneDx
03.2022 - 03.2023
- Prepared and maintained laboratory supplies and reagents, ensuring all samples are properly stored and documented according to GLP and CLIA standards.
- Prepared patient samples for genetic analysis, ensuring proper labeling, documentation, and sample tracking in LIMS.
- Assisted in the calibration and maintenance of laboratory equipment to ensure accuracy and reliability of test results.
- Troubleshooted minor issues with laboratory equipment and assist in resolving any workflow disruptions.
Line & Prep
Café Rio
07.2020 - 02.2022
- Operated the point-of-sale (POS) system to accurately process customer orders and handle cash transactions.
- Delivered exceptional customer service by greeting customers, taking orders, and addressing inquiries or concerns in a timely manner.
- Followed food safety and sanitation guidelines, maintaining a clean work area and practicing proper food handling.
- Collaborated with kitchen and front-of-house staff to ensure efficient service during peak hours.
- Assisted with inventory control, ensuring that ingredients are fresh and well-stocked to meet customer demand.
- Trained and mentored new team members, providing guidance on food prep, safety standards, and customer service.
Child Care Aide
Carl & Norma Miller Children's Center
08.2018 - 08.2019
- Assisted in supervising a group of children, ensuring a safe and positive environment for play and learning.
- Assisted with the development of personalized learning activities to enhance children’s cognitive and motor skills.
- Helped children with basic skills such as tying shoes, washing hands, and using the restroom.
- Supported children in building social skills through group activities and cooperative play.
- Observed children's behavior and reported any unusual occurrences to senior staff.
- Provided emotional and physical support to children in need, offering comfort during nap time or when upset.
- Maintained daily logs for each child, including meals, naps, and any incidents.
Education
Bachelor of Science - Neuroscience
University of Maryland, College Park
College Park, MD12-2021
Associate of Science - STEM
Frederick Community College
Frederick, MD05-2019
Skills
- Cytogenetic Analysis: Fluorescence in situ hybridization (FISH)
- Molecular Biology Techniques: PCR, qPCR, DNA/RNA extraction
- Laboratory Instruments: Fluorescence microscopes, automated pipetting systems, incubators
- Bioinformatics Tools: LIMS (Laboratory Information Management Systems)
- Regulatory Knowledge: CLIA, CAP, GLP standards, HIPAA compliance
- Data Analysis: Interpretation of genetic results
- Team Collaboration & Communication: Coordinating with multidisciplinary teams for accurate genetic diagnosis, and to ensure that SOPs are aligned with departmental needs, best practices, and regulatory compliance
- Bilingual: Fluent in Spanish
- Aseptic Technique: Performing cell culture procedures with strict adherence to aseptic protocols, including media preparation, sub-culturing, and maintaining cell lines in sterile conditions
Timeline
Cytogenetics Technologist II
GeneDx
03.2023 - Current
Cytogenetics Technician I & II
GeneDx
03.2022 - 03.2023
Line & Prep
Café Rio
07.2020 - 02.2022
Child Care Aide
Carl & Norma Miller Children's Center
08.2018 - 08.2019
Bachelor of Science - Neuroscience
University of Maryland, College Park
Associate of Science - STEM
Frederick Community College
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