Dushyant Kumar Gautam

Passionate biomedical researcher specializing in hematopoiesis, immunology, and molecular biology. Postdoctoral Fellow at Johns Hopkins University with a focus on hematopoietic regulation, inflammatory pathways, and disease modeling. Made significant contributions to the understanding of immune signaling, specifically the Toll/NF-κB pathway, in hematological disorders. Holds a Ph.D. in Biochemistry from the University of Hyderabad, bringing expertise in molecular cloning, gene expression analysis, CRISPR/Cas9 genome editing, and advanced microscopy. Recognized for dedication to the field through prestigious fellowships (DBT-JRF, ICMR-SRF) and multiple awards. Strong publication record in peer-reviewed journals. Committed to mentoring, interdisciplinary collaboration, and advancing novel therapeutic strategies.

• Aquib Siddique, Zeeshan Ahmad, 2020, 2022, M.Sc. Batch
• Srivani, 2021, Project Student
• Shouvik Bhattacharya, 2021, 2023, M.Sc. Batch

• Workshop on Mendeley, University of Hyderabad, Hyderabad, INDIA, 03/09/17
• Animal Models in Cancer Research, School of Life Sciences, University of Hyderabad, 10/24/17, 10/25/17
• Workshop on Research and Publication Ethics, University of Hyderabad, Hyderabad, INDIA, 10/24/18

• Awarded gold medal in M.Sc. Biotechnology tenure (Category), University of Hyderabad, INDIA
• Received travel award for 24th International Congress of APBMT & ICBMT, Busan, South Korea, 08/01/19
• Best poster presentation award in Bio-Quest 2016, University of Hyderabad, 2016
• Contribution award for poster presentation at ICKSH 2021, Seoul, SOUTH KOREA, 03/01/21
• Poster exhibition award at International Congress of BMT 2021, SOUTH KOREA, 08/01/21

• Methotrexate suppresses constitutively activated Toll pathway in immune tissues by regulating Insulin signaling, International Conference on Virus Evolution, Infection and Disease Control (ICVEIDC), Hyderabad, INDIA, 12/15/22, 12/17/22

• Methotrexate (MTX) mitigates the wasp induced Toll/NF-kB response in the larval stage of Drosophila melanogaster, 48th Annual Conference of Indian Immunology Society, Banaras Hindu University (BHU), Varanasi, INDIA, 07/08/22, 07/09/22

• Methotrexate hinders the expression of Drosomycin induced via Toll/NF-kB pathway in both acute and chronic inflammation, 13th International Symposium on Antimicrobial Agents and Resistance (ISAAR), Seoul, SOUTH KOREA, 09/09/21, 09/11/21

• Effects of Methotrexate on larval hematopoiesis in Drosophila melanogaster, 5th Asia-Pacific Drosophila Research Conference (APDRC5) and 4th Indian Drosophila Research Conference (InDRC), IISER Pune, INDIA, 01/06/20, 01/10/20

• Effects of Methotrexate on larval hematopoiesis in Drosophila melanogaster, XLIII All India Cell Biology Conference (AICBC), IISER Mohali, INDIA, 12/19/19, 12/21/19

• Effects of Methotrexate (MTX) on larval hematopoiesis in Drosophila melanogaster, Silver jubilee (25th Year) celebration of Department of Biochemistry University of Hyderabad, INDIA, 09/26/19, 09/27/19

• Studying the effects of Methotrexate on hematopoiesis and NF-kB pathway using in vitro and in vivo model systems, 24th International Congress of APBMT& ICBMT Korean Society of Haematology International Conference, Busan, SOUTH KOREA, 08/30/19, 09/01/19

• Effect of Methotrexate (MTX) on hematopoietic pathways in Drosophila melanogaster, Bio-Quest 2019 "Cellular Homeostasis: From the Basics to Translational Research", University of Hyderabad, INDIA, 03/11/19

• 3rd Indian Drosophila Research Conference (InDRC), IISER Bhopal, INDIA, 12/06/17, 12/09/17

• Methotrexate (MTX) suppresses immune response in Drosophila melanogaster larva against parasitoid wasp (Leptopilina boulardi 17) infestation, Bio-Quest 2016 "The Welcome Trust/DBT India Alliance", University of Hyderabad, INDIA, 10/20/16, 10/21/16

• 82nd Annual meeting of The Society of Biological Chemists (India) and International Conference on "Genomes: Mechanism and Function", Hyderabad, INDIA, 12/02/13, 12/05/13

• Methotrexate (MTX) restores hematopoietic equilibrium by affecting the nutritional signaling in the FAT body via Toll/NF-kB Pathway in Drosophila larval hematopoiesis, Gautam DK, Willem, Ahmad Z, Gutti RK, Paddibhatla I, Journal of Cell Science, Undergoing first revision, 2025,

• Methotrexate negatively acts on inflammatory responses triggered in Drosophila larva with hyperactive JAK/STAT pathway, Yadav RK, Gautam DK, Muj C, Gajula Balija MB, Paddibhatla I, Dev Comp Immunol, 10/01/21, 10.1016/j.dci.2021.104161,

• Comparative hematopoiesis and signal transduction in model organisms, Gautam DK, Chimata AV, Gutti RK, Paddibhatla I, J Cell Physiol, 08/01/21, 10.1002/jcp.30287,

• SETDB1 modulates the differentiation of both the crystal cells and the lamellocytes in Drosophila, Paddibhatla I, Gautam DK, Mishra RK, Dev Biol, 12/01/19, 10.1016/j.ydbio.2019.08.008,

• Virodhamine, an endocannabinoid, induces megakaryocyte differentiation by regulating MAPK activity and function of mitochondria, Sharma DS, Raghuwanshi S, Kovuru N, Dahariya S, Gautam DK, Paddibhatla I, Gutti RK, J Cell Physiol, 02/01/21, 10.1002/jcp.29949,

• Endocannabinoid system: Role in blood cell development, neuroimmune interactions and associated disorders, Sharma DS, Paddibhatla I, Raghuwanshi S, Malleswarapu M, Sangeeth A, Kovuru N, Dahariya S, Gautam DK, Pallepati A, Gutti RK, J Neuroimmunol, 04/15/21, 10.1016/j.jneuroim.2021.577501,

• MicroRNA function in megakaryocytes, Raghuwanshi S, Dahariya S, Musvi SS, Gutti U, Kandi R, Undi RB, Sahu I, Gautam DK, Paddibhatla I, Gutti RK, Platelets, 2019, 10.1080/09537104.2018.1528343,

• Chapter 2: Investigative Tools to Study Blood Cells: A Focus on Single Cell Isolation and Analysis, Gautam DK, Anuradha Venkatakrishnan Chimata, Ravi Kumar Gutti and Indira Paddibhatla, Hematopoiesis: Subtitle: Biochemical Cellular, Molecular and Genomic Perspectives. doi: 10.1201/9781003413059, ISBN: 9781003413059, CRC Press Taylor & Francis Group, Publisher: Apple Academic Press

• Chapter 10: LncRNAs in Leukemia, Hematopoiesis, Cellular, Molecular and Genomic Perspectives, CRC Press Taylor & Francis Group, Swati Dahariya, Gautam DK, Aditya Velidandi, Ravi Kumar Gutti and Indira Paddibhatla Hematopoiesis: Subtitle: Biochemical Cellular, Molecular and Genomic Perspectives. doi: 10.1201/9781003413059, ISBN: 9781003413059, CRC Press Taylor & Francis Group, Publisher: Apple Academic Press

• Manuscript in Preparation: Role of SETDB1 in HOX9A regulation in leukemia in-vitro and in-vivo, Gautam DK, Bhattacharya S, Nivetha, Ayamanns I, Gutti RK,

• Manuscript in Preparation: SETDB1 maintains the inflammatory-immune axis between the blood cells and the fat body by regulating HOX genes in Drosophila larva, Paddibhatla I, Gautam DK, Gutti RK

• Qualified Graduate Aptitude Test in Engineering (GATE)- Life Sciences, 03/27/15, INDIA
• Qualified Department of Biotechnology- Junior Research Fellowship (DBT-JRF) Category-II BET Examination, 05/01/15, INDIA
• Two years Combined Entrance Examination for Biotechnology, JNU (CEEB scholarship), 07/01/13, 05/31/15, INDIA
• Three years study scholarship, government of U.P., 07/01/09, 06/30/12, INDIA
• Awarded Junior Research Fellowship by Indian Council of Medical Research (ICMR), New Delhi, 08/01/17, INDIA
• Upgraded to Senior Research Fellowship by Indian Council of Medical Research (ICMR), New Delhi, 08/01/19, INDIA

• Aquib Siddique, Zeeshan Ahmad, 2020, 2022, M.Sc. Batch
• Srivani, 2021, Project Student
• Shouvik Bhattacharya, 2021, 2023, M.Sc. Batch

• Workshop on Mendeley, University of Hyderabad, Hyderabad, INDIA, 03/09/17
• Animal Models in Cancer Research, School of Life Sciences, University of Hyderabad, 10/24/17, 10/25/17
• Workshop on Research and Publication Ethics, University of Hyderabad, Hyderabad, INDIA, 10/24/18

• Awarded gold medal in M.Sc. Biotechnology tenure (Category), University of Hyderabad, INDIA
• Received travel award for 24th International Congress of APBMT & ICBMT, Busan, South Korea, 08/01/19
• Best poster presentation award in Bio-Quest 2016, University of Hyderabad, 2016
• Contribution award for poster presentation at ICKSH 2021, Seoul, SOUTH KOREA, 03/01/21
• Poster exhibition award at International Congress of BMT 2021, SOUTH KOREA, 08/01/21

• Methotrexate suppresses constitutively activated Toll pathway in immune tissues by regulating Insulin signaling, International Conference on Virus Evolution, Infection and Disease Control (ICVEIDC), Hyderabad, INDIA, 12/15/22, 12/17/22

• Methotrexate (MTX) mitigates the wasp induced Toll/NF-kB response in the larval stage of Drosophila melanogaster, 48th Annual Conference of Indian Immunology Society, Banaras Hindu University (BHU), Varanasi, INDIA, 07/08/22, 07/09/22

• Methotrexate hinders the expression of Drosomycin induced via Toll/NF-kB pathway in both acute and chronic inflammation, 13th International Symposium on Antimicrobial Agents and Resistance (ISAAR), Seoul, SOUTH KOREA, 09/09/21, 09/11/21

• Effects of Methotrexate on larval hematopoiesis in Drosophila melanogaster, 5th Asia-Pacific Drosophila Research Conference (APDRC5) and 4th Indian Drosophila Research Conference (InDRC), IISER Pune, INDIA, 01/06/20, 01/10/20

• Effects of Methotrexate on larval hematopoiesis in Drosophila melanogaster, XLIII All India Cell Biology Conference (AICBC), IISER Mohali, INDIA, 12/19/19, 12/21/19

• Effects of Methotrexate (MTX) on larval hematopoiesis in Drosophila melanogaster, Silver jubilee (25th Year) celebration of Department of Biochemistry University of Hyderabad, INDIA, 09/26/19, 09/27/19

• Studying the effects of Methotrexate on hematopoiesis and NF-kB pathway using in vitro and in vivo model systems, 24th International Congress of APBMT& ICBMT Korean Society of Haematology International Conference, Busan, SOUTH KOREA, 08/30/19, 09/01/19

• Effect of Methotrexate (MTX) on hematopoietic pathways in Drosophila melanogaster, Bio-Quest 2019 "Cellular Homeostasis: From the Basics to Translational Research", University of Hyderabad, INDIA, 03/11/19

• 3rd Indian Drosophila Research Conference (InDRC), IISER Bhopal, INDIA, 12/06/17, 12/09/17

• Methotrexate (MTX) suppresses immune response in Drosophila melanogaster larva against parasitoid wasp (Leptopilina boulardi 17) infestation, Bio-Quest 2016 "The Welcome Trust/DBT India Alliance", University of Hyderabad, INDIA, 10/20/16, 10/21/16

• 82nd Annual meeting of The Society of Biological Chemists (India) and International Conference on "Genomes: Mechanism and Function", Hyderabad, INDIA, 12/02/13, 12/05/13

• Methotrexate (MTX) restores hematopoietic equilibrium by affecting the nutritional signaling in the FAT body via Toll/NF-kB Pathway in Drosophila larval hematopoiesis, Gautam DK, Willem, Ahmad Z, Gutti RK, Paddibhatla I, Journal of Cell Science, Undergoing first revision, 2025,

• Methotrexate negatively acts on inflammatory responses triggered in Drosophila larva with hyperactive JAK/STAT pathway, Yadav RK, Gautam DK, Muj C, Gajula Balija MB, Paddibhatla I, Dev Comp Immunol, 10/01/21, 10.1016/j.dci.2021.104161,

• Comparative hematopoiesis and signal transduction in model organisms, Gautam DK, Chimata AV, Gutti RK, Paddibhatla I, J Cell Physiol, 08/01/21, 10.1002/jcp.30287,

• SETDB1 modulates the differentiation of both the crystal cells and the lamellocytes in Drosophila, Paddibhatla I, Gautam DK, Mishra RK, Dev Biol, 12/01/19, 10.1016/j.ydbio.2019.08.008,

• Virodhamine, an endocannabinoid, induces megakaryocyte differentiation by regulating MAPK activity and function of mitochondria, Sharma DS, Raghuwanshi S, Kovuru N, Dahariya S, Gautam DK, Paddibhatla I, Gutti RK, J Cell Physiol, 02/01/21, 10.1002/jcp.29949,

• Endocannabinoid system: Role in blood cell development, neuroimmune interactions and associated disorders, Sharma DS, Paddibhatla I, Raghuwanshi S, Malleswarapu M, Sangeeth A, Kovuru N, Dahariya S, Gautam DK, Pallepati A, Gutti RK, J Neuroimmunol, 04/15/21, 10.1016/j.jneuroim.2021.577501,

• MicroRNA function in megakaryocytes, Raghuwanshi S, Dahariya S, Musvi SS, Gutti U, Kandi R, Undi RB, Sahu I, Gautam DK, Paddibhatla I, Gutti RK, Platelets, 2019, 10.1080/09537104.2018.1528343,

• Chapter 2: Investigative Tools to Study Blood Cells: A Focus on Single Cell Isolation and Analysis, Gautam DK, Anuradha Venkatakrishnan Chimata, Ravi Kumar Gutti and Indira Paddibhatla, Hematopoiesis: Subtitle: Biochemical Cellular, Molecular and Genomic Perspectives. doi: 10.1201/9781003413059, ISBN: 9781003413059, CRC Press Taylor & Francis Group, Publisher: Apple Academic Press

• Chapter 10: LncRNAs in Leukemia, Hematopoiesis, Cellular, Molecular and Genomic Perspectives, CRC Press Taylor & Francis Group, Swati Dahariya, Gautam DK, Aditya Velidandi, Ravi Kumar Gutti and Indira Paddibhatla Hematopoiesis: Subtitle: Biochemical Cellular, Molecular and Genomic Perspectives. doi: 10.1201/9781003413059, ISBN: 9781003413059, CRC Press Taylor & Francis Group, Publisher: Apple Academic Press

• Manuscript in Preparation: Role of SETDB1 in HOX9A regulation in leukemia in-vitro and in-vivo, Gautam DK, Bhattacharya S, Nivetha, Ayamanns I, Gutti RK,

• Manuscript in Preparation: SETDB1 maintains the inflammatory-immune axis between the blood cells and the fat body by regulating HOX genes in Drosophila larva, Paddibhatla I, Gautam DK, Gutti RK